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8*15k dna microarray  (Agilent technologies)


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    Structured Review

    Agilent technologies 8*15k dna microarray
    8*15k Dna Microarray, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/8*15k+dna+microarray/pmc04414249-216-2-8
    Average 90 stars, based on 1 article reviews
    8*15k dna microarray - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Multiple Colonization with S. pneumoniae before and after Introduction of the Seven-Valent Conjugated Pneumococcal Polysaccharide Vaccine
    Article Snippet: Briefly, DNA samples were fluorescently labelled and hybridized to the Agilent 8×15K format microarray according to manufacturer's instructions for the Agilent genomic DNA enzymatic labelling and oligo aCGH hybridisation reagent kits.

    Article Title: Promoter variants for protein production
    Article Snippet: In-house designed P. pastoris specific oligonucleotide arrays (AMAD-ID: 026594, 8×15K custom arrays, Agilent) were used.

    Article Title: Metabolic Reprogramming of Clostridioides difficile During the Stationary Phase With the Induction of Toxin Production
    Article Snippet: A customized whole-genome DNA microarray (8 × 15K format; Agilent, Santa Clara, CA, United States) of the genes of C. difficile 630Δ erm was designed with the eArray platform from Agilent according to recently published revised genome sequence ( ; ).

    Article Title: Recombinant host cell for expressing proteins of interest
    Article Snippet: In-house designed P. pastoris specific oligonucleotide arrays (AMAD-ID: 034821, 8×15K custom arrays, Agilent) were used.

    Article Title: An oligodendrocyte silencer element underlies the pathogenic impact of lamin B1 structural variants
    Article Snippet: Array CGH was performed at the University of Pittsburgh on genomic DNA from clinical samples, hybridized on a custom 8×15K HD-CGH microarray previously described , scanned in an G2565CA Agilent microarray scanner, and analyzed using Agilent CGH Analytics software (Agilent Technologies).

    Microarray:

    Article Title: SPINE: SParse eIgengene NEtwork Linking Gene Expression Clusters in Dehalococcoides mccartyi to Perturbations in Experimental Conditions
    Article Snippet: .. The microarray data used to construct the SPINE resulted from the scan of an Agilent two-color 8 × 15k oligonucleotide array that captured Cy3 and Cy5 labeled reverse-transcribed complimentary DNA (cDNA) (Agilent Technologies, Santa Clara, CA). ..

    Article Title: Ploidy influences cellular responses to gross chromosomal rearrangements in saccharomyces cerevisiae
    Article Snippet: .. Agilent DNA microarray slides (GE 8 × 15K n AMADID 015761) containing probes for most of the yeast open reading frames were used. ..

    Article Title: FibroChip, a Functional DNA Microarray to Monitor Cellulolytic and Hemicellulolytic Activities of Rumen Microbiota
    Article Snippet: .. Triplicate of probes of 25- and 54-mers were synthetized in situ on an Agilent 8 × 15K DNA microarray (Agilent Technologies, Santa Clara, CA, United States) allowing the simultaneous analysis of 16 biological samples using a two color labeling. .. The microarray contained also 382 Agilent internal control probes including positive controls, negative controls, and quality control probes.

    Construct:

    Article Title: SPINE: SParse eIgengene NEtwork Linking Gene Expression Clusters in Dehalococcoides mccartyi to Perturbations in Experimental Conditions
    Article Snippet: .. The microarray data used to construct the SPINE resulted from the scan of an Agilent two-color 8 × 15k oligonucleotide array that captured Cy3 and Cy5 labeled reverse-transcribed complimentary DNA (cDNA) (Agilent Technologies, Santa Clara, CA). ..

    Labeling:

    Article Title: SPINE: SParse eIgengene NEtwork Linking Gene Expression Clusters in Dehalococcoides mccartyi to Perturbations in Experimental Conditions
    Article Snippet: .. The microarray data used to construct the SPINE resulted from the scan of an Agilent two-color 8 × 15k oligonucleotide array that captured Cy3 and Cy5 labeled reverse-transcribed complimentary DNA (cDNA) (Agilent Technologies, Santa Clara, CA). ..

    Article Title: FibroChip, a Functional DNA Microarray to Monitor Cellulolytic and Hemicellulolytic Activities of Rumen Microbiota
    Article Snippet: .. Triplicate of probes of 25- and 54-mers were synthetized in situ on an Agilent 8 × 15K DNA microarray (Agilent Technologies, Santa Clara, CA, United States) allowing the simultaneous analysis of 16 biological samples using a two color labeling. .. The microarray contained also 382 Agilent internal control probes including positive controls, negative controls, and quality control probes.

    Reverse Transcription:

    Article Title: SPINE: SParse eIgengene NEtwork Linking Gene Expression Clusters in Dehalococcoides mccartyi to Perturbations in Experimental Conditions
    Article Snippet: .. The microarray data used to construct the SPINE resulted from the scan of an Agilent two-color 8 × 15k oligonucleotide array that captured Cy3 and Cy5 labeled reverse-transcribed complimentary DNA (cDNA) (Agilent Technologies, Santa Clara, CA). ..

    In Situ:

    Article Title: FibroChip, a Functional DNA Microarray to Monitor Cellulolytic and Hemicellulolytic Activities of Rumen Microbiota
    Article Snippet: .. Triplicate of probes of 25- and 54-mers were synthetized in situ on an Agilent 8 × 15K DNA microarray (Agilent Technologies, Santa Clara, CA, United States) allowing the simultaneous analysis of 16 biological samples using a two color labeling. .. The microarray contained also 382 Agilent internal control probes including positive controls, negative controls, and quality control probes.



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    Image Search Results


     Microarray  results obtained by hybridization of seven cloned genes encoding different CAZymes from diverse microorganisms targeted by the FibroChip.

    Journal: Frontiers in Microbiology

    Article Title: FibroChip, a Functional DNA Microarray to Monitor Cellulolytic and Hemicellulolytic Activities of Rumen Microbiota

    doi: 10.3389/fmicb.2018.00215

    Figure Lengend Snippet: Microarray results obtained by hybridization of seven cloned genes encoding different CAZymes from diverse microorganisms targeted by the FibroChip.

    Article Snippet: Triplicate of probes of 25- and 54-mers were synthetized in situ on an Agilent 8 × 15K DNA microarray (Agilent Technologies, Santa Clara, CA, United States) allowing the simultaneous analysis of 16 biological samples using a two color labeling.

    Techniques: Microarray, Hybridization, Clone Assay

     Microarray  results obtained after hybridization of genomic  DNA  from selected bacterial strains.

    Journal: Frontiers in Microbiology

    Article Title: FibroChip, a Functional DNA Microarray to Monitor Cellulolytic and Hemicellulolytic Activities of Rumen Microbiota

    doi: 10.3389/fmicb.2018.00215

    Figure Lengend Snippet: Microarray results obtained after hybridization of genomic DNA from selected bacterial strains.

    Article Snippet: Triplicate of probes of 25- and 54-mers were synthetized in situ on an Agilent 8 × 15K DNA microarray (Agilent Technologies, Santa Clara, CA, United States) allowing the simultaneous analysis of 16 biological samples using a two color labeling.

    Techniques: Microarray, Hybridization

    Microarray gene expression analysis of the targeted genes from Fibrobacter succinogenes S85 cultivated with cellobiose (Clb) or wheat straw (WS) as a sole substrate. Relative expression of expressed genes on WS versus Clb is expressed as log2 ratio (WS/Clb) ± SEM. Genes are denominated by the accession number of the protein encoded.

    Journal: Frontiers in Microbiology

    Article Title: FibroChip, a Functional DNA Microarray to Monitor Cellulolytic and Hemicellulolytic Activities of Rumen Microbiota

    doi: 10.3389/fmicb.2018.00215

    Figure Lengend Snippet: Microarray gene expression analysis of the targeted genes from Fibrobacter succinogenes S85 cultivated with cellobiose (Clb) or wheat straw (WS) as a sole substrate. Relative expression of expressed genes on WS versus Clb is expressed as log2 ratio (WS/Clb) ± SEM. Genes are denominated by the accession number of the protein encoded.

    Article Snippet: Triplicate of probes of 25- and 54-mers were synthetized in situ on an Agilent 8 × 15K DNA microarray (Agilent Technologies, Santa Clara, CA, United States) allowing the simultaneous analysis of 16 biological samples using a two color labeling.

    Techniques: Microarray, Expressing

    Bacterial strains, plasmids, and primers used in this study

    Journal: Infection and Immunity

    Article Title: Transcriptome Analysis of Avian Pathogenic Escherichia coli O1 in Chicken Serum Reveals Adaptive Responses to Systemic Infection

    doi: 10.1128/IAI.01230-10

    Figure Lengend Snippet: Bacterial strains, plasmids, and primers used in this study

    Article Snippet: The 8 × 15,000 (15K) DNA high-density microarray of APEC O1 was designed by Oxford Gene Technology (Oxford OX5 1PF, United Kingdom) and validated by the University of Birmingham E. coli Centre (UBEC) (United Kingdom).

    Techniques: Plasmid Preparation, Sequencing, Mutagenesis, Real-time Polymerase Chain Reaction

    Growth of APEC O1 in LB and chicken serum. Optical density (OD600) (A) or CFU counts (B) were used to assess the growth of APEC O1 in culture medium LB (□) and chicken serum (▪). APEC O1 grew faster in serum than in LB, suggesting that the APEC O1 can rapidly adapt to chicken serum.

    Journal: Infection and Immunity

    Article Title: Transcriptome Analysis of Avian Pathogenic Escherichia coli O1 in Chicken Serum Reveals Adaptive Responses to Systemic Infection

    doi: 10.1128/IAI.01230-10

    Figure Lengend Snippet: Growth of APEC O1 in LB and chicken serum. Optical density (OD600) (A) or CFU counts (B) were used to assess the growth of APEC O1 in culture medium LB (□) and chicken serum (▪). APEC O1 grew faster in serum than in LB, suggesting that the APEC O1 can rapidly adapt to chicken serum.

    Article Snippet: The 8 × 15,000 (15K) DNA high-density microarray of APEC O1 was designed by Oxford Gene Technology (Oxford OX5 1PF, United Kingdom) and validated by the University of Birmingham E. coli Centre (UBEC) (United Kingdom).

    Techniques:

    Heat map visualizing regulated genes in APEC O1 grown in chicken serum compared to growth in LB. Genes found to be significantly regulated are indicated by either green (upregulation) or red (downregulation). Significantly differentially expressed genes of APEC O1 during growth in serum compared to that in LB are listed in columns 2 and 3. The genes are sorted by functional categories (column 1) according to annotation by the J. Craig Venter Institute.

    Journal: Infection and Immunity

    Article Title: Transcriptome Analysis of Avian Pathogenic Escherichia coli O1 in Chicken Serum Reveals Adaptive Responses to Systemic Infection

    doi: 10.1128/IAI.01230-10

    Figure Lengend Snippet: Heat map visualizing regulated genes in APEC O1 grown in chicken serum compared to growth in LB. Genes found to be significantly regulated are indicated by either green (upregulation) or red (downregulation). Significantly differentially expressed genes of APEC O1 during growth in serum compared to that in LB are listed in columns 2 and 3. The genes are sorted by functional categories (column 1) according to annotation by the J. Craig Venter Institute.

    Article Snippet: The 8 × 15,000 (15K) DNA high-density microarray of APEC O1 was designed by Oxford Gene Technology (Oxford OX5 1PF, United Kingdom) and validated by the University of Birmingham E. coli Centre (UBEC) (United Kingdom).

    Techniques: Functional Assay

    Significantly upregulated genes of  APEC O1  localized in genomic islands that are not present in E. coli K-12

    Journal: Infection and Immunity

    Article Title: Transcriptome Analysis of Avian Pathogenic Escherichia coli O1 in Chicken Serum Reveals Adaptive Responses to Systemic Infection

    doi: 10.1128/IAI.01230-10

    Figure Lengend Snippet: Significantly upregulated genes of APEC O1 localized in genomic islands that are not present in E. coli K-12

    Article Snippet: The 8 × 15,000 (15K) DNA high-density microarray of APEC O1 was designed by Oxford Gene Technology (Oxford OX5 1PF, United Kingdom) and validated by the University of Birmingham E. coli Centre (UBEC) (United Kingdom).

    Techniques: Expressing, Binding Assay

    Significantly upregulated genes from plasmids of  APEC O1  cultured in chicken serum compared to results with LB

    Journal: Infection and Immunity

    Article Title: Transcriptome Analysis of Avian Pathogenic Escherichia coli O1 in Chicken Serum Reveals Adaptive Responses to Systemic Infection

    doi: 10.1128/IAI.01230-10

    Figure Lengend Snippet: Significantly upregulated genes from plasmids of APEC O1 cultured in chicken serum compared to results with LB

    Article Snippet: The 8 × 15,000 (15K) DNA high-density microarray of APEC O1 was designed by Oxford Gene Technology (Oxford OX5 1PF, United Kingdom) and validated by the University of Birmingham E. coli Centre (UBEC) (United Kingdom).

    Techniques: Cell Culture, Expressing, Plasmid Preparation

    Comparison of gene regulation analyzed by microarray (filled) or real-time quantitative RT-PCR (open). Real-time quantitative RT-PCR was used to validate the expression level for 11 selected genes, including 6 upregulated genes and 4 downregulated genes revealed by microarray analysis.

    Journal: Infection and Immunity

    Article Title: Transcriptome Analysis of Avian Pathogenic Escherichia coli O1 in Chicken Serum Reveals Adaptive Responses to Systemic Infection

    doi: 10.1128/IAI.01230-10

    Figure Lengend Snippet: Comparison of gene regulation analyzed by microarray (filled) or real-time quantitative RT-PCR (open). Real-time quantitative RT-PCR was used to validate the expression level for 11 selected genes, including 6 upregulated genes and 4 downregulated genes revealed by microarray analysis.

    Article Snippet: The 8 × 15,000 (15K) DNA high-density microarray of APEC O1 was designed by Oxford Gene Technology (Oxford OX5 1PF, United Kingdom) and validated by the University of Birmingham E. coli Centre (UBEC) (United Kingdom).

    Techniques: Comparison, Microarray, Quantitative RT-PCR, Expressing